Jiang, Kai Liu, Yajuan Fan, Junkai Zhang, Jie Li, Xiang-An Evers, B. Mark Zhu, Haining Jia, Jianhang Kinase activity-independent role of Gprk2 by enriching PI(4)P in vivo. <p>(A–C) Wing discs carrying <i>gprk2</i> clones and expressing Flag-Gprk2<sup>WT</sup>, Flag-Gprk2<sup>ΔC</sup>, or Flag-Gprk2-PH<sup>OSBP</sup> by <i>MS1096</i>-Gal4 were immunostained for En and GFP. The <i>gprk2</i> mutant clones were marked by the lack of GFP expression. Arrows indicate the mutant clones near the A/P boundary. (D–F’) Wing discs expressing Flag-Gprk2<sup>WT</sup> by the dorsal compartment-specific <i>ap</i>-Gal4 were treated with 100 μM PI(4)P (with 1:1 Carrier 3) and 0.1 μg/mL ecdysone in the M3 medium for 4 h and then immunostained for Smo, PI(4)P, and Flag. Dashed lines indicate the dorsal/ventral boundary marked by immunostaining with the anti-Flag antibody, which is not included here. Arrows indicate elevated levels of Smo and PI(4)P by the expression of Gprk2<sup>WT</sup>. (G–I) Wing discs expressing Flag-Gprk2<sup>ΔC</sup> by <i>ap</i>-Gal4 were treated with PI(4)P as described above. Dashed lines indicate the dorsal/ventral boundaries.</p> Hh stimulation;triggers Smo phosphorylation;Hh treatment increases;pleckstrin homology;arginine motif;mouse Smo;ph;pi;Smo activation;Ptc inhibition;ciliary accumulation;Conformational Change 2016-02-10
    https://plos.figshare.com/articles/figure/_Kinase_activity_independent_role_of_Gprk2_by_enriching_PI_4_P_in_vivo_/1643827
10.1371/journal.pbio.1002375.g004