Sathler-Avelar, Renato Marquete Vitelli-Avelar, Danielle Moreira Mattoso-Barbosa, Armanda Perdigão-de-Oliveira, Marcelo Peres Costa, Ronaldo Maria Elói-Santos, Silvana de Souza Gomes, Matheus Rodrigues do Amaral, Laurence Teixeira-Carvalho, Andréa Assis Martins-Filho, Olindo J. Dick Jr, Edward B. Hubbard, Gene F. VandeBerg, Jane L. VandeBerg, John Systems biology strategy for analyzing adaptive immunity flow-cytometry data by heatmap and decision-tree analysis. <p>(A) Bioinformatics tool applied for single-cell data mining using heatmap computational method to preprocess flow cytometry data and to identify the adaptive immunity cell attributes. (B) Decision tree analysis identifies “root” (CD3<sup>+</sup>HLA-DR<sup>+</sup>) and “secondary” (CD8<sup>+</sup>HLA-DR<sup>+</sup> and CD8<sup>+</sup> Granzyme A<sup>+</sup>) cell attributes with higher accuracy to distinguish between non-human primates naturally infected with <i>T</i>. <i>cruzi</i> and non-infected controls. (C) Scatter distribution plots show the potential of selected biomarkers to discriminate infected from non-infected individuals. White rectangles indicate true positive (Chagas disease) and true negative (non-infected subjects) classifications. Gray rectangles indicate subjects that require the analysis of additional characteristics for accurate classification by the algorithm sequence proposed by the decision tree. (C) ROC curve analysis illustrating the cut-off points, the global accuracy (area under the curve–AUC) and performance indexes (sensitivity–Se, specificity–Sp and likelihood ratio–LR) for each selected biomarker.</p> Trypanosoma cruzi Resemble;Systems Biology Approaches;systems biology analysis;expression;Human Chagas Disease BackgroundCynomolgus macaques;ni;nk;15 cynomolgus macaques;Chagas disease;ch;Major Immunological Findings Observed;granzyme;imbricate biomarker networks 2016-01-28
    https://plos.figshare.com/articles/figure/_Systems_biology_strategy_for_analyzing_adaptive_immunity_flow_cytometry_data_by_heatmap_and_decision_tree_analysis_/1642036
10.1371/journal.pntd.0004302.g005