Serologic and perturbed gene expression in time-shifted data. C. LaughlinRichard L. DrakeKenneth C. MorrillJohn Garry AdamsL. 2016 <p>(<b>A</b>) Log10 plaque reducing neutralizing titers (Log10(PRNT<sub>80</sub>)) of test calves prior to and after vaccination with arMP-12 with data synchronized to the time point that each animal reached the minimum threshold (T = 0; 1:80, log10 = 1.903). (<b>B</b>) Schematic illustrating the immunologic status of test animals in time shifted analysis: unvaccinated (gray), vaccinated, below PRNT<sub>80</sub> cutoff (light red), vaccinated, first day at or above PRNT<sub>80</sub> cutoff (dark green), vaccinated, above PRNT<sub>80</sub> (light green). (<b>C</b>) Count of significantly perturbed genes normalized against unvaccinated samples occurring in at least 50% of animals by time pre-serum neutralization, with a |Z-score ≥2.24| and a false discovery rate (FDR) ≤0.05.</p>