Specificities mediating neutralization breadth and potency in the top 42 Protocol C neutralizers.
Elise Landais
Xiayu Huang
Colin Havenar-Daughton
Ben Murrell
Matt A. Price
Lalinda Wickramasinghe
Alejandra Ramos
Charoan B. Bian
Melissa Simek
Susan Allen
Etienne Karita
William Kilembe
Shabir Lakhi
Mubiana Inambao
Anatoli Kamali
Eduard J. Sanders
Omu Anzala
Vinodh Edward
Linda-Gail Bekker
Jianming Tang
Jill Gilmour
Sergei L. Kosakovsky-Pond
Pham Phung
Terri Wrin
Shane Crotty
Adam Godzik
Pascal Poignard
10.1371/journal.ppat.1005369.g004
https://plos.figshare.com/articles/figure/_Specificities_mediating_neutralization_breadth_and_potency_in_the_top_42_Protocol_C_neutralizers_/1637686
<p>(A) Samples are ranked by their neutralization score on the 37-virus panel (37vP) (S4 Fig in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a> and S2 Table in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a>). VC: Visit Code (months post infection). Symbols recapitulate the strength of the phenotypes tested using the different approaches detailed in this manuscript (S7-S11 Figures in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a>) to determine the Env epitope region targeted by the plasma broadly neutralizing activity: gp120 absorption of bnAb activity, effect of b6 competition in gp120 absorption experiments, RSC3 binding and neutralization competition, HIV-2 chimera neutralization, viruses produced in presence of kifunensine or bearing mutations. Absent (-), very weak (+/-), weak (+), moderate (++), strong (+++), phenotype was attributed based on i) the median fold or average percent decrease in ID50 and ii) the fraction of viruses which neutralization ID50 was decreased <2 fold, <10 <50 fold or <20%, <40%, <60%, <80%. Blank = not tested, NB: not binding. A dominant specificity was attributed for each sample based on results from all these experiments. UD: Undefined. (B) Overall distribution of dominant nAb specificities mediating plasma neutralization breadth in the top 42 Protocol C neutralizers as detailed in (A).</p>
2016-01-28 12:36:33
neutralization breadth
IAVI Protocol C program
bnAb responses
vaccine
infection
plasma IgG levels
CD 4 T cell counts
hiv
neutralizing antibody responses