Specificities mediating neutralization breadth and potency in the top 42 Protocol C neutralizers. Elise Landais Xiayu Huang Colin Havenar-Daughton Ben Murrell Matt A. Price Lalinda Wickramasinghe Alejandra Ramos Charoan B. Bian Melissa Simek Susan Allen Etienne Karita William Kilembe Shabir Lakhi Mubiana Inambao Anatoli Kamali Eduard J. Sanders Omu Anzala Vinodh Edward Linda-Gail Bekker Jianming Tang Jill Gilmour Sergei L. Kosakovsky-Pond Pham Phung Terri Wrin Shane Crotty Adam Godzik Pascal Poignard 10.1371/journal.ppat.1005369.g004 https://plos.figshare.com/articles/figure/_Specificities_mediating_neutralization_breadth_and_potency_in_the_top_42_Protocol_C_neutralizers_/1637686 <p>(A) Samples are ranked by their neutralization score on the 37-virus panel (37vP) (S4 Fig in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a> and S2 Table in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a>). VC: Visit Code (months post infection). Symbols recapitulate the strength of the phenotypes tested using the different approaches detailed in this manuscript (S7-S11 Figures in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005369#ppat.1005369.s001" target="_blank">S1 Text</a>) to determine the Env epitope region targeted by the plasma broadly neutralizing activity: gp120 absorption of bnAb activity, effect of b6 competition in gp120 absorption experiments, RSC3 binding and neutralization competition, HIV-2 chimera neutralization, viruses produced in presence of kifunensine or bearing mutations. Absent (-), very weak (+/-), weak (+), moderate (++), strong (+++), phenotype was attributed based on i) the median fold or average percent decrease in ID50 and ii) the fraction of viruses which neutralization ID50 was decreased <2 fold, <10 <50 fold or <20%, <40%, <60%, <80%. Blank = not tested, NB: not binding. A dominant specificity was attributed for each sample based on results from all these experiments. UD: Undefined. (B) Overall distribution of dominant nAb specificities mediating plasma neutralization breadth in the top 42 Protocol C neutralizers as detailed in (A).</p> 2016-01-28 12:36:33 neutralization breadth IAVI Protocol C program bnAb responses vaccine infection plasma IgG levels CD 4 T cell counts hiv neutralizing antibody responses