4-MU4-deoxy-xyloside reduced TGF-β1-induced increased expression and actvation of endogenous TGF-β1.
Irfan Shaukat
Lydia Barré
Narayanan Venkatesan
Dong Li
Jean-Claude Jaquinet
Sylvie Fournel-Gigleux
Mohamed Ouzzine
10.1371/journal.pone.0146499.g008
https://plos.figshare.com/articles/figure/_4_MU4_deoxy_xyloside_reduced_TGF_946_1_induced_increased_expression_and_actvation_of_endogenous_TGF_946_1_/1635606
<p><b>(A)</b> Lung fibroblasts were treated with 500 μM of 4-MU4-deoxy-xyloside and/or TGF-β1 (5 ng/ml) for 6 h and the expression of endogenous TGF-β1was analyzed by qPCR. The relative expression was normalized with ribosomal protein S29. Data are expressed as mean ± SD of three separate experiments, (*P<0.05). <b>(B)</b> Fibroblasts were treated with 500 μM of 4-MU4-deoxy-xyloside and/or TGF-β1 (5 ng/ml) for 24 h and the total and active TGF-β1 levels were measured by ELISA in cell culture supernatants as described in Materials and methods section.</p>
2016-01-18 15:12:56
attenuates profibrotic effects
Counteract Excessive Matrix Proteoglycan Deposition
tgf
Proteoglycan Synthesis Pathway
mu
lung fibroblasts
matrix PG deposition
fibrosis
Lung Fibroblasts Stimulation
PG synthesis