Shaukat, Irfan Barré, Lydia Venkatesan, Narayanan Li, Dong Jaquinet, Jean-Claude Fournel-Gigleux, Sylvie Ouzzine, Mohamed 4-MU4-deoxy-xyloside counteracts TGF-β1-induced fibroblast-to-myofibroblast trans-differentiation. <p>Lung fibroblasts were treated with 500 μM of 4-MU4-deoxy-xyloside and/or TGF-β1 (5 ng/ml) for 24 h and the expression of αSMA was analyzed by qPCR (<b>A</b>) and by Western Blot (<b>B</b>). The relative expression was normalized with ribosomal protein S29. Data are expressed as mean ± SD of three separate experiments; *P<0.05 versus control. For Western blot, whole cell lysates were analysed αSMA content. β-actin was used as a loading control. <b>(C)</b> Bar graph shows the expression of αSMA relative to β-actin level and expressed relative to control lysate. Data are expressed as mean ± SD of three separate experiments; *P<0.05 versus control.</p> attenuates profibrotic effects;Counteract Excessive Matrix Proteoglycan Deposition;tgf;Proteoglycan Synthesis Pathway;mu;lung fibroblasts;matrix PG deposition;fibrosis;Lung Fibroblasts Stimulation;PG synthesis 2016-01-18
    https://plos.figshare.com/articles/figure/_4_MU4_deoxy_xyloside_counteracts_TGF_946_1_induced_fibroblast_to_myofibroblast_trans_differentiation_/1635605
10.1371/journal.pone.0146499.g007