10.1371/journal.pone.0145918.g010
Natalya Panova
Natalya
Panova
Eva Zborníková
Eva
Zborníková
Ondřej Šimák
Ondřej
Šimák
Radek Pohl
Radek
Pohl
Milan Kolar
Milan
Kolar
Katerina Bogdanova
Katerina
Bogdanova
Renata Vecerova
Renata
Vecerova
Gabriela Seydlová
Gabriela
Seydlová
Radovan Fiser
Radovan
Fiser
Romana Hadravová
Romana
Hadravová
Hana Šanderová
Hana
Šanderová
Dragana Vítovská
Dragana
Vítovská
Michaela Šiková
Michaela
Šiková
Tomáš Látal
Tomáš
Látal
Petra Lovecká
Petra
Lovecká
Ivan Barvík
Ivan
Barvík
Libor Krásný
Libor
Krásný
Dominik Rejman
Dominik
Rejman
<i>B</i>. <i>subtilis</i> 168 develops resistance against rifampicin (rif) but not against DR5026.
Public Library of Science
2016
strain
transepithelial transport
Bactericidal Lipophosphonoxins
cell disintegration
Ames test
bactericidal concentrations LPPOs
compound
plasmatic membrane
acidic environment
2016-01-06 09:33:23
Figure
https://plos.figshare.com/articles/figure/_B_subtilis_168_develops_resistance_against_rifampicin_rif_but_not_against_DR5026_/1630222
<p><i>B</i>. <i>subtilis</i> was incubated with subcytotoxic concentrations of rif (starting at 0.01 mg/L; MIC 0.06 mg/L) and <b>DR5026</b> (0.5 mg/L MIC ~ 3 mg/L) and grown for 24 h. Then, aliquots of the cultures were transferred to new tubes with fresh medium and a two-fold increased concentration of the active compound. A binary representation is shown; 1 indicates growth of the cells, i. e. their resistance to the respective compound; 0 represents lack of growth—the cells were sensitive to the compound and no resistant cell appeared within the time frame of the experiment. The experiment was conducted in three biological replicates with the same results.</p>