10.1371/journal.pone.0145918.g010 Natalya Panova Natalya Panova Eva Zborníková Eva Zborníková Ondřej Šimák Ondřej Šimák Radek Pohl Radek Pohl Milan Kolar Milan Kolar Katerina Bogdanova Katerina Bogdanova Renata Vecerova Renata Vecerova Gabriela Seydlová Gabriela Seydlová Radovan Fiser Radovan Fiser Romana Hadravová Romana Hadravová Hana Šanderová Hana Šanderová Dragana Vítovská Dragana Vítovská Michaela Šiková Michaela Šiková Tomáš Látal Tomáš Látal Petra Lovecká Petra Lovecká Ivan Barvík Ivan Barvík Libor Krásný Libor Krásný Dominik Rejman Dominik Rejman <i>B</i>. <i>subtilis</i> 168 develops resistance against rifampicin (rif) but not against DR5026. Public Library of Science 2016 strain transepithelial transport Bactericidal Lipophosphonoxins cell disintegration Ames test bactericidal concentrations LPPOs compound plasmatic membrane acidic environment 2016-01-06 09:33:23 Figure https://plos.figshare.com/articles/figure/_B_subtilis_168_develops_resistance_against_rifampicin_rif_but_not_against_DR5026_/1630222 <p><i>B</i>. <i>subtilis</i> was incubated with subcytotoxic concentrations of rif (starting at 0.01 mg/L; MIC 0.06 mg/L) and <b>DR5026</b> (0.5 mg/L MIC ~ 3 mg/L) and grown for 24 h. Then, aliquots of the cultures were transferred to new tubes with fresh medium and a two-fold increased concentration of the active compound. A binary representation is shown; 1 indicates growth of the cells, i. e. their resistance to the respective compound; 0 represents lack of growth—the cells were sensitive to the compound and no resistant cell appeared within the time frame of the experiment. The experiment was conducted in three biological replicates with the same results.</p>