10.1371/journal.pbio.1002316.g003 Danae Schulz Danae Schulz Monica R. Mugnier Monica R. Mugnier Eda-Margaret Paulsen Eda-Margaret Paulsen Hee-Sook Kim Hee-Sook Kim Chun-wa W. Chung Chun-wa W. Chung David F. Tough David F. Tough Inmaculada Rioja Inmaculada Rioja Rab K. Prinjha Rab K. Prinjha F. Nina Papavasiliou F. Nina Papavasiliou Erik W. Debler Erik W. Debler Bromodomain proteins are necessary for BF-specific antibody internalization. Public Library of Science 2015 Bloodstream Form Stage Identity novel binding mode immunogenic surface VSG African Trypanosome Trypanosoma brucei bromodomain inhibition host antibody responses 1.25Å resolution crystal structure BF cell fate bromodomain proteins trypanosome bromodomain proteins bloodstream stage identity immunogenic Variant Surface Glycoprotein 2015-12-08 12:47:16 Dataset https://plos.figshare.com/articles/dataset/_Bromodomain_proteins_are_necessary_for_BF_specific_antibody_internalization_/1621516 <p>(A) Flow cytometry measuring the amount of primary anti-VSG antibody remaining on the surface following a 5-min incubation at the indicated temperatures, washout, fixation, and incubation with secondary antibody in I-BET151-treated (right) versus control cells (left). "Secondary" (gray trace) denotes control cells stained with secondary antibody only. (B) Immunofluorescence showing the amount of primary anti-VSG antibody remaining on the surface following a 5-min incubation at the indicated temperatures, washout, fixation, and incubation with secondary antibody in I-BET151-treated versus control cells. A bright spot is detected at the flagellar pocket in control cells incubated at 4°C, whereas I-BET151-treated cells have antibody distributed over the entire surface. At 37°C, control cells have internalized almost all the primary antibody. (C) Flow cytometry showing the amount of anti-VSG3 antibody bound to the surface in cells treated with I-BET151 for 3 d, compared to control cells. (D) Anti-VSG Western blot showing that protein levels of <i>VSG</i> at the active expression site (ES) are unaltered in I-BET151-treated cells. Tubulin is used as a loading control. Numerical data for Fig 3 is in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002316#pbio.1002316.s003" target="_blank">S3 Data</a>.</p>