10.1371/journal.pbio.1002316.g003
Danae Schulz
Danae
Schulz
Monica R. Mugnier
Monica
R. Mugnier
Eda-Margaret Paulsen
Eda-Margaret
Paulsen
Hee-Sook Kim
Hee-Sook
Kim
Chun-wa W. Chung
Chun-wa
W. Chung
David F. Tough
David
F. Tough
Inmaculada Rioja
Inmaculada
Rioja
Rab K. Prinjha
Rab K.
Prinjha
F. Nina Papavasiliou
F.
Nina Papavasiliou
Erik W. Debler
Erik W.
Debler
Bromodomain proteins are necessary for BF-specific antibody internalization.
Public Library of Science
2015
Bloodstream Form Stage Identity
novel binding mode
immunogenic surface VSG
African Trypanosome Trypanosoma brucei
bromodomain inhibition
host antibody responses
1.25Å resolution crystal structure
BF cell fate
bromodomain proteins
trypanosome bromodomain proteins
bloodstream stage identity
immunogenic Variant Surface Glycoprotein
2015-12-08 12:47:16
Dataset
https://plos.figshare.com/articles/dataset/_Bromodomain_proteins_are_necessary_for_BF_specific_antibody_internalization_/1621516
<p>(A) Flow cytometry measuring the amount of primary anti-VSG antibody remaining on the surface following a 5-min incubation at the indicated temperatures, washout, fixation, and incubation with secondary antibody in I-BET151-treated (right) versus control cells (left). "Secondary" (gray trace) denotes control cells stained with secondary antibody only. (B) Immunofluorescence showing the amount of primary anti-VSG antibody remaining on the surface following a 5-min incubation at the indicated temperatures, washout, fixation, and incubation with secondary antibody in I-BET151-treated versus control cells. A bright spot is detected at the flagellar pocket in control cells incubated at 4°C, whereas I-BET151-treated cells have antibody distributed over the entire surface. At 37°C, control cells have internalized almost all the primary antibody. (C) Flow cytometry showing the amount of anti-VSG3 antibody bound to the surface in cells treated with I-BET151 for 3 d, compared to control cells. (D) Anti-VSG Western blot showing that protein levels of <i>VSG</i> at the active expression site (ES) are unaltered in I-BET151-treated cells. Tubulin is used as a loading control. Numerical data for Fig 3 is in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002316#pbio.1002316.s003" target="_blank">S3 Data</a>.</p>