Fajardo Jr., Teodoro Sung, Po-Yu Roy, Polly Effect of ORN on RNA packaging in <i>cell-free</i> assembly assay. <p><sup>35</sup>S-labelled <i>in vitro</i> assembled BTV complexes were fractionated in a continuous sucrose gradient [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005321#ppat.1005321.ref006" target="_blank">6</a>]. (Upper panel): Fractions #5, #6 and #7 from <i>cell-free</i> assembly (CFA) reactions in the absence (+control, lanes 1 to 3) or presence of 20 pmol S10.2 ORN (lanes 4 to 6) alongside with fraction #6 in the presence of S10.4 ORN (lane 7) S10.5 (lane 8) and Scr ORN (lane 9) were analysed on 1% denaturing agarose gel. Packaged RNAs were determined by densitometry. Lower panel represents the mean values (%) of total packaged ssRNAs in the presence of ORNs calculated relative to the control reaction (without ORNs) set at 100% (n = 3). The synthesized <sup>35</sup>S-labelled BTV subcore and transcription complex protein profile are shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005321#ppat.1005321.s008" target="_blank">S8 Fig</a>.</p> novel RNA-RNA interaction assay;supramolecular complexes;substitution mutations;double-stranded RNA molecules;Virus Infectivity Bluetongue virus;UTR;BTV;Double-Stranded RNA Virus Inhibits Genome Packaging;Putative packaging signals;Specific RNA-RNA Interactions;virus replication;virus capsid assembly;causes hemorrhagic disease;ORN;1-S;sequential packaging pathway;RNA packaging sequence 2015-12-08
    https://plos.figshare.com/articles/figure/_Effect_of_ORN_on_RNA_packaging_in_cell_free_assembly_assay_/1621510
10.1371/journal.ppat.1005321.g006