Qian, Guofeng Fan, Wei Ahlemeyer, Barbara Karnati, Srikanth Baumgart-Vogt, Eveline Activation of PPAR<i>ß</i> increased the peroxisome number and metabolic function in MC3T3-E1 cells. <p>(A) Comparative analysis of the mRNA levels (qRT-PCR) of <i>Pparɑ</i>, <i>Pparß</i> and <i>Ppar</i><b><i>ɣ</i></b>in MC3T3-E1 cells. (B) MC3T3-E1 cells were treated with the six PPAR-modulating drugs. PPRE-activity was measured using the Dual Luciferase Reporter Gene Assay. Significant differences in comparison to untreated controls were given as *p≤0.05; **p≤0.01 and ***p≤0.001 using ANOVA-1 followed by post-hoc Scheffé-test. (C-H) Treatment of MC3T3-E1 cells with the PPARß agonist GW0742 (D, G) increased the number of peroxisomes as detected by immunofluorescence stainings for PEX14 (C-E) and PEX13 (F-H) in comparison to cells treated with vehicle (control; C, F) and the PPARß antagonist GSK0660 (E, H). G. Semiquantitative RT-PCR analysis of genes regulating peroxisome number (<i>Pex11)</i> as well as peroxisome biogenesis (<i>Pex13</i>) and metabolic function (<i>Cat</i>, <i>Acox1</i>) after treatment of MC3T3-E1 cells with GW0742.</p> PPAR ß;PPAR ɣ. Treatment;knockout mouse models;peroxisomal genes;M 3CT cells;PPAR ß antagonist GSK 0660;gene expression;MC 3T cells;osteoblast differentiation;peroxisome number;calvarial osteoblasts;cell types;PPAR family members;peroxisomal compartment;Pex 11β gene expression;gene expression patterns;ppre;PPAR ß agonist GW 0742 2015-12-02
    https://plos.figshare.com/articles/figure/_Activation_of_PPAR_223_increased_the_peroxisome_number_and_metabolic_function_in_MC3T3_E1_cells_/1616988
10.1371/journal.pone.0143439.g009