10.1371/journal.pone.0143439.g004 Guofeng Qian Guofeng Qian Wei Fan Wei Fan Barbara Ahlemeyer Barbara Ahlemeyer Srikanth Karnati Srikanth Karnati Eveline Baumgart-Vogt Eveline Baumgart-Vogt The peroxisome numerical abundance increased during osteoblast differentiation. Public Library of Science 2015 PPAR ß PPAR ɣ. Treatment knockout mouse models peroxisomal genes M 3CT cells PPAR ß antagonist GSK 0660 gene expression MC 3T cells osteoblast differentiation peroxisome number calvarial osteoblasts cell types PPAR family members peroxisomal compartment Pex 11β gene expression gene expression patterns ppre PPAR ß agonist GW 0742 2015-12-02 04:10:53 Figure https://plos.figshare.com/articles/figure/_The_peroxisome_numerical_abundance_increased_during_osteoblast_differentiation_/1616983 <p>(A) In primary osteoblast cultures (day 12) from the calvariae of newborn mice, more than 95% of the cells were positively stained for the early osteoblast marker OPN. (B) The formation of calcium nodules increased in primary osteoblasts during culture as shown by Alizarin red staining of the Petri dishes. (C-L) Immunofluorescence stainings of osteoblasts at different time points during culture detecting cell proliferation using anti-Ki67 antibodies (C-G) and peroxisome numerical abundance using anti-PEX14 antibodies (H-L). Nuclei were visualized with Hoechst 33342 (Hoechst, C-F). Representative images (C-F, H-K) and quantitative analysis (G, L) are shown.</p>