10.1371/journal.pone.0143439.g004
Guofeng Qian
Guofeng
Qian
Wei Fan
Wei
Fan
Barbara Ahlemeyer
Barbara
Ahlemeyer
Srikanth Karnati
Srikanth
Karnati
Eveline Baumgart-Vogt
Eveline
Baumgart-Vogt
The peroxisome numerical abundance increased during osteoblast differentiation.
Public Library of Science
2015
PPAR ß
PPAR ɣ. Treatment
knockout mouse models
peroxisomal genes
M 3CT cells
PPAR ß antagonist GSK 0660
gene expression
MC 3T cells
osteoblast differentiation
peroxisome number
calvarial osteoblasts
cell types
PPAR family members
peroxisomal compartment
Pex 11β gene expression
gene expression patterns
ppre
PPAR ß agonist GW 0742
2015-12-02 04:10:53
Figure
https://plos.figshare.com/articles/figure/_The_peroxisome_numerical_abundance_increased_during_osteoblast_differentiation_/1616983
<p>(A) In primary osteoblast cultures (day 12) from the calvariae of newborn mice, more than 95% of the cells were positively stained for the early osteoblast marker OPN. (B) The formation of calcium nodules increased in primary osteoblasts during culture as shown by Alizarin red staining of the Petri dishes. (C-L) Immunofluorescence stainings of osteoblasts at different time points during culture detecting cell proliferation using anti-Ki67 antibodies (C-G) and peroxisome numerical abundance using anti-PEX14 antibodies (H-L). Nuclei were visualized with Hoechst 33342 (Hoechst, C-F). Representative images (C-F, H-K) and quantitative analysis (G, L) are shown.</p>