%0 Figure %A Crenshaw, Ezekiel %A P. Leung, Brian %A Kwok, Chun Kit %A Sharoni, Michal %A Olson, Kalee %A P. Sebastian, Neeraj %A Ansaloni, Sara %A Schweitzer-Stenner, Reinhard %A R. Akins, Michael %A Bevilacqua, Philip C. %A J. Saunders, Aleister %D 2015 %T G-quadruplex regulation of APP gene expression. %U https://plos.figshare.com/articles/figure/_G_quadruplex_regulation_of_APP_gene_expression_/1614088 %R 10.1371/journal.pone.0143160.g004 %2 https://plos.figshare.com/ndownloader/files/2594207 %K amyloid precursor protein %K Amyloid Precursor Protein Translation %K APP protein expression %K APP expression results %K nm %K pcr %K APP expression %K modulating APP levels %K equivalent mRNA levels %K Western Blot analysis %K utr %X

(A) Western blot analysis of cells transfected with reporter constructs containing APP 695 coding sequence with wild type (G-quad WT) or mutant (G-quad Mut) sequence. Mock Transfection was used to confirm that these constructs were over-expressed in HeLa cells. Antibody C1/6.1 recognizes both full-length APP695 and CTF. β-Actin was used as a loading control. (B-C) Quantification of Western blots as in 4A with (B) APP levels normalized to β-Actin and (C) Endogenous CTF intensity values were subtracted from total CTF intensity values to obtain exogenous CTF values which were then normalized to β-Actin values. (D) Aβ ELISA quantification of total Aβ levels from conditioned medium of cells transfected with APP constructs containing the wild type or mutant 3’UTR G-quadruplex.

%I PLOS ONE