G-quadruplex regulation of APP gene expression. Ezekiel Crenshaw Brian P. Leung Chun Kit Kwok Michal Sharoni Kalee Olson Neeraj P. Sebastian Sara Ansaloni Reinhard Schweitzer-Stenner Michael R. Akins Philip C. Bevilacqua Aleister J. Saunders 10.1371/journal.pone.0143160.g004 https://plos.figshare.com/articles/figure/_G_quadruplex_regulation_of_APP_gene_expression_/1614088 <p>(A) Western blot analysis of cells transfected with reporter constructs containing APP <sub>695</sub> coding sequence with wild type (G-quad WT) or mutant (G-quad Mut) sequence. Mock Transfection was used to confirm that these constructs were over-expressed in HeLa cells. Antibody C1/6.1 recognizes both full-length APP<sub>695</sub> and CTF. β-Actin was used as a loading control. (B-C) Quantification of Western blots as in 4A with (B) APP levels normalized to β-Actin and (C) Endogenous CTF intensity values were subtracted from total CTF intensity values to obtain exogenous CTF values which were then normalized to β-Actin values. (D) Aβ ELISA quantification of total Aβ levels from conditioned medium of cells transfected with APP constructs containing the wild type or mutant 3’UTR G-quadruplex.</p> 2015-11-30 02:57:11 amyloid precursor protein Amyloid Precursor Protein Translation APP protein expression APP expression results nm pcr APP expression modulating APP levels equivalent mRNA levels Western Blot analysis utr