G-quadruplex regulation of APP gene expression.
Ezekiel Crenshaw
Brian P. Leung
Chun Kit Kwok
Michal Sharoni
Kalee Olson
Neeraj P. Sebastian
Sara Ansaloni
Reinhard Schweitzer-Stenner
Michael R. Akins
Philip C. Bevilacqua
Aleister J. Saunders
10.1371/journal.pone.0143160.g004
https://plos.figshare.com/articles/figure/_G_quadruplex_regulation_of_APP_gene_expression_/1614088
<p>(A) Western blot analysis of cells transfected with reporter constructs containing APP <sub>695</sub> coding sequence with wild type (G-quad WT) or mutant (G-quad Mut) sequence. Mock Transfection was used to confirm that these constructs were over-expressed in HeLa cells. Antibody C1/6.1 recognizes both full-length APP<sub>695</sub> and CTF. β-Actin was used as a loading control. (B-C) Quantification of Western blots as in 4A with (B) APP levels normalized to β-Actin and (C) Endogenous CTF intensity values were subtracted from total CTF intensity values to obtain exogenous CTF values which were then normalized to β-Actin values. (D) Aβ ELISA quantification of total Aβ levels from conditioned medium of cells transfected with APP constructs containing the wild type or mutant 3’UTR G-quadruplex.</p>
2015-11-30 02:57:11
amyloid precursor protein
Amyloid Precursor Protein Translation
APP protein expression
APP expression results
nm
pcr
APP expression
modulating APP levels
equivalent mRNA levels
Western Blot analysis
utr