10.1371/journal.pone.0143509.g002 Mette Elena Skindersoe Mette Elena Skindersoe Karen A. Krogfelt Karen A. Krogfelt Ashley Blom Ashley Blom Guowei Jiang Guowei Jiang Glenn D. Prestwich Glenn D. Prestwich Jason Peter Mansell Jason Peter Mansell Growth and maturation of human (MG63) osteoblasts in response to 16:0 OMPT and 1,25D. Public Library of Science 2015 LPA analogues offer Methicillin Resistant Staphylococcus aureus Titanium mg TJR implant Ti surface 2015-11-25 04:14:59 Figure https://plos.figshare.com/articles/figure/_Growth_and_maturation_of_human_MG63_osteoblasts_in_response_to_16_0_OMPT_and_1_25D_/1613207 <p><b>A.</b> MG63 osteoblasts were treated with 1,25D (100nM), 16:0 OMPT (micromolarμM) or a combination of these agents and left for three days under conventional cell culturing conditions prior to an assessment of cell growth. As anticipated for the pro-differentiating effects of 1,25D, there were fewer cells (*p<0.01 1,25D versus control) at the end of the culture period. In contrast the application of 16:0 OMPT led to a significant increase in cell numbers (**p<0.01 16:0 OMPT versus control). <b>B.</b> Alkaline phosphatase (ALP) is expressed in greater abundance as osteoblasts progress from an immature to a more differentiated phenotype. Enzyme activity is reliably monitored via the hydrolysis of p-nitrophenyl phosphate to p-nitrophenol (p-NP). The co-stimulation of MG63 cells with 100nM 1,25D and 16:0 OMPT yielded significant increases in total ALP activity by 72 h (*p < 0.001 versus agents in isolation). In each case the data shown are the mean + SD of 4 replicate samples and are a representative from three independent experiments.</p>