10.1371/journal.pone.0143467.g003
Jie Zhu
Jie
Zhu
Binbin Wang
Binbin
Wang
Qiuhong Miao
Qiuhong
Miao
Yonggui Tan
Yonggui
Tan
Chuanfeng Li
Chuanfeng
Li
Zongyan Chen
Zongyan
Chen
Huimin Guo
Huimin
Guo
Guangqing Liu
Guangqing
Liu
The interaction between VPg and eIF4E in RK13 cells based on the mammalian two-hybrid assay.
Public Library of Science
2015
novel cap substitute
Rabbit hemorrhagic disease virus
RHDV translation
vpg
tissue culture system
eukaryotic initiation factor 4 E
rabbit hemorrhagic disease
eIF 4E
rk
RHDV replicon
2015-11-23 15:22:07
Figure
https://plos.figshare.com/articles/figure/_The_interaction_between_VPg_and_eIF4E_in_RK13_cells_based_on_the_mammalian_two_hybrid_assay_/1610228
<p>RK13 cells (2×10<sup>5</sup> cells per well in a 6-well plate) were co-transfected with the pACT (1 μg) and pBIND (1 μg) plasmids, pACT-VPg (1 μg) and pBIND (1 μg) plasmids, and pACT-VPg (1 μg) and pBIND-eIF4E (1 μg) plasmids, respectively. RK13 cells co-transfected with the pACT-MyoD (1 μg) and pBIND-ID (1 μg) plasmids served as positive controls [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143467#pone.0143467.ref025" target="_blank">25</a>]. A blank control was also used. In addition, all of the groups were transfected with pGL4.75 (0.2 μg). The cells were lysed at 48 h post-transfection, and Fluc activity was evaluated based on RLUs and normalized according to the results obtained for a co-transfected plasmid encoding the Renilla luciferase. The experiments were conducted in triplicate, and similar results were obtained from three independent experiments. The Student’s <i>t</i> test and ANOVA were applied for the statistical analyses, <i>P</i> <0.01 was considered as extremely significant different (**).</p>