10.1371/journal.pone.0143063.g004 Wafa Markikou-Ouni Wafa Markikou-Ouni Sima Drini Sima Drini Narges Bahi-Jaber Narges Bahi-Jaber Mehdi Chenik Mehdi Chenik Amel Meddeb-Garnaoui Amel Meddeb-Garnaoui Effect of LipESP on LPS-, IFN-γ-, or IFN-γ/LPS-induced cytokine production by human monocytes. Public Library of Science 2015 CD 40 expression CD 86 surface expression maturation T cell activation lps monocyte LiP 15 LipESP Human Dendritic Cells Differentiation DCs differentiation elongation factor 1 protein Maturation Leishmania parasites tnf il CD 1a LiP 23 ifn Leishmania infantum 2015-11-18 03:36:00 Figure https://plos.figshare.com/articles/figure/_Effect_of_LipESP_on_LPS_IFN_947_or_IFN_947_LPS_induced_cytokine_production_by_human_monocytes_/1606806 <p>Production of (a) IL-12p70, (b) IL-10 and (c) TNF-α were measured in monocytes supernatant by ELISA after 24 h of stimulation by LipESP in presence or absence of LPS (for IL-10 and TNF-α analysis) or after 12 h of priming with IFN-γ followed by 24 additional hours of stimulation by LipESP with or without LPS co-treatment (for IL-12 analysis). Results are expressed as mean ± standard deviation (n = 9). * Statistically significant difference between non stimulated and LipESP-, LPS-, and IFN-γ/LPS-stimulated monocytes (p<0·05); ** Statistically significant difference between IFN-γ stimulated and LipESP/IFN-γ-co-treated monocytes (p<0·05) for IL-12p70 and between LPS-stimulated and LipESP-LPS-co-treated monocytes (p<0·05) for IL-10 and TNF-α; *** Statistically significant difference between IFN-γ/LPS stimulated and LipESP/IFN-γ/LPS-co-treated monocytes (p<0·05).</p>