15-HETE-induced ROS promote PASMC cell cycle progression and α-tubulin polymerization in the nucleus.

After employing NAC to scavenge ROS in PASMCs, the effects of hypoxia and exogenous 15-HETE on (A) the percentages of cells in the S and G2/M phases were analyzed by flow cytometry (n = 3). (B) α-tubulin polymerization in the nucleus was detected by immunocytochemistry. Scale bars indicate 100 μm. Data are presented as the means ± SEM; *p<0.05, **p<0.01 versus normal; # p<0.05, ##p<0.01 versus Hyp+15 (n = 3). Nor, normoxia; Hyp, hypoxia; 15, 15-HETE.