%0 Figure %A Liu, Jia %A Bai, Jing %A Jiang, Guoqiang %A Li, Xinli %A Wang, Jing %A Wu, Dachang %A Owusu, Lawrence %A Zhang, Ershao %A Li, Weiling %D 2015 %T PMBPs suppress MMP-9 activity and expression. %U https://plos.figshare.com/articles/figure/_PMBPs_suppress_MMP_9_activity_and_expression_/1595168 %R 10.1371/journal.pone.0142157.g007 %2 https://plos.figshare.com/ndownloader/files/2409000 %K p 38 MAPK %K activation %K Pinus massoniana bark %K cancer cells %K PMBP %K Pinus massoniana Bark Proanthocyanidins %K transwell chamber assays %K Cell Migration Pinus massoniana bark proanthocyanidins %K nf %K mmp %K erk %X

The cells were treated with PMBPs (0, 5, 10, 25 μg/ml) for 24 h. (A) Activity of MMP-9 in the cell supernatant at various concentrations of PMBPs was examined by gelatin zymography assay. The white bands represent MMP-9 mediated gelatin digestion. (B) The protein expression of MMP-9 in A2780 cells at various concentrations of PMBPs was evaluated by Western blot. Histogram shows mean level of MMP-9 (±SD) from three independent experiments. MMP-9 protein level was expressed relative to loading control, β-Actin, and standardized to the non-treated control group. *P<0.05 and **P<0.01 compared to the control group.

%I PLOS ONE