PMBPs suppress MMP-9 activity and expression.
Jia Liu
Jing Bai
Guoqiang Jiang
Xinli Li
Jing Wang
Dachang Wu
Lawrence Owusu
Ershao Zhang
Weiling Li
10.1371/journal.pone.0142157.g007
https://plos.figshare.com/articles/figure/_PMBPs_suppress_MMP_9_activity_and_expression_/1595168
<p>The cells were treated with PMBPs (0, 5, 10, 25 μg/ml) for 24 h. (A) Activity of MMP-9 in the cell supernatant at various concentrations of PMBPs was examined by gelatin zymography assay. The white bands represent MMP-9 mediated gelatin digestion. (B) The protein expression of MMP-9 in A2780 cells at various concentrations of PMBPs was evaluated by Western blot. Histogram shows mean level of MMP-9 (±SD) from three independent experiments. MMP-9 protein level was expressed relative to loading control, β-Actin, and standardized to the non-treated control group. *<i>P<0</i>.<i>05</i> and **<i>P<0</i>.<i>01</i> compared to the control group.</p>
2015-11-05 03:38:14
p 38 MAPK
activation
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PMBP
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erk