10.1371/journal.pone.0142157.g007 Jia Liu Jia Liu Jing Bai Jing Bai Guoqiang Jiang Guoqiang Jiang Xinli Li Xinli Li Jing Wang Jing Wang Dachang Wu Dachang Wu Lawrence Owusu Lawrence Owusu Ershao Zhang Ershao Zhang Weiling Li Weiling Li PMBPs suppress MMP-9 activity and expression. Public Library of Science 2015 p 38 MAPK activation Pinus massoniana bark cancer cells PMBP Pinus massoniana Bark Proanthocyanidins transwell chamber assays Cell Migration Pinus massoniana bark proanthocyanidins nf mmp erk 2015-11-05 03:38:14 Figure https://plos.figshare.com/articles/figure/_PMBPs_suppress_MMP_9_activity_and_expression_/1595168 <p>The cells were treated with PMBPs (0, 5, 10, 25 μg/ml) for 24 h. (A) Activity of MMP-9 in the cell supernatant at various concentrations of PMBPs was examined by gelatin zymography assay. The white bands represent MMP-9 mediated gelatin digestion. (B) The protein expression of MMP-9 in A2780 cells at various concentrations of PMBPs was evaluated by Western blot. Histogram shows mean level of MMP-9 (±SD) from three independent experiments. MMP-9 protein level was expressed relative to loading control, β-Actin, and standardized to the non-treated control group. *<i>P<0</i>.<i>05</i> and **<i>P<0</i>.<i>01</i> compared to the control group.</p>