Deletions suppressing cell lysis in Δ<i>ura4</i> strains grown on YPD medium. Kohei Nishino Misaki Kushima Yuzy Matsuo Yasuhiro Matsuo Makoto Kawamukai 10.1371/journal.pone.0141796.g001 https://plos.figshare.com/articles/figure/_Deletions_suppressing_cell_lysis_in_ura4_strains_grown_on_YPD_medium_/1594584 <p> (A) L972 (<i>ura4</i><sup>+</sup>), UMP31 (Δ<i>ura4</i>), KT35 (<i>ura4</i>-<i>D18</i>), and indicated deletion mutants (Bioneer deletion library ver4.0) were grown for 12 h, then spotted onto YPD medium and incubated for 3 days at 30°C. For the alkaline phosphatase assay, YPD plates were overlaid with a phosphatase assay solution containing 50 mM glycine-NaOH (pH 9.8), 1% agar, and 2.5 mg/ml of BCIP for 30 min. (B) Cells were also observed by microscopy after 3 days of growth on YPD. Five examples of mutants exhibiting different levels of lysis suppression are shown. Bar: 10 μm.</p> 2015-11-04 06:21:23 E 3 ubiquitin ligase Δ ura 4 strains cell lysis Δ ura 4 cells Uracil uptake activity pombe ura 4 Mutants ump pub 1 gene plasma membrane fur 4 Δ ura 4 background Δ pub 1 cells Δ pub 1 strain Uracil Transporter Fur 4 Schizosaccharomyces pombe Δ ura 4 cells lyse ypd uracil uptake ability