Secretion of TGFβig-h3 is stimulated by chemerin and IGF. J. Dinesh Kumar Chris Holmberg Silvia Balabanova Lyudmyla Borysova Ted Burdyga Robert Beynon Graham J. Dockray Andrea Varro 10.1371/journal.pone.0141331.g001 https://plos.figshare.com/articles/figure/_Secretion_of_TGF_946_ig_h3_is_stimulated_by_chemerin_and_IGF_/1589258 <p><i>A</i>. Western blot analysis of TGFβig-h3 in media from MSC cells shows stimulation by chemerin and IGF-II and inhibition by CCX832 and AG1042, respectively. <i>B</i>. Stimulated secretion of TGFβig-h3 is maintained after cycloheximide treatment. TGFβig-h3 abundance in cell extracts was unchanged (middle panel); GAPDH was used as a loading control for the cell extracts (bottom panel). <i>C</i>. The calcium ionophore, ionomycin (1μM) stimulated TGFβig-h3 secretion comparable to IGF-I (50ng.ml<sup>-1</sup>) and IGF-II (100ng.ml<sup>-1</sup>). <i>D</i>. In calcium-free medium stimulated secretion in response to IGF-II is inhibited.</p> 2015-10-29 03:25:18 TGF βig adhesion receptor tyrosine kinase stimulant exocytosi igf Mesenchymal Stem Cells Exhibit Regulated Exocytosis secretory protein transport secretion chemerin mmp gene ontology analysis exhibit preformed secretory vesicles msc