10.1371/journal.pone.0141331.g001 J. Dinesh Kumar J. Dinesh Kumar Chris Holmberg Chris Holmberg Silvia Balabanova Silvia Balabanova Lyudmyla Borysova Lyudmyla Borysova Ted Burdyga Ted Burdyga Robert Beynon Robert Beynon Graham J. Dockray Graham J. Dockray Andrea Varro Andrea Varro Secretion of TGFβig-h3 is stimulated by chemerin and IGF. Public Library of Science 2015 TGF βig adhesion receptor tyrosine kinase stimulant exocytosi igf Mesenchymal Stem Cells Exhibit Regulated Exocytosis secretory protein transport secretion chemerin mmp gene ontology analysis exhibit preformed secretory vesicles msc 2015-10-29 03:25:18 Figure https://plos.figshare.com/articles/figure/_Secretion_of_TGF_946_ig_h3_is_stimulated_by_chemerin_and_IGF_/1589258 <p><i>A</i>. Western blot analysis of TGFβig-h3 in media from MSC cells shows stimulation by chemerin and IGF-II and inhibition by CCX832 and AG1042, respectively. <i>B</i>. Stimulated secretion of TGFβig-h3 is maintained after cycloheximide treatment. TGFβig-h3 abundance in cell extracts was unchanged (middle panel); GAPDH was used as a loading control for the cell extracts (bottom panel). <i>C</i>. The calcium ionophore, ionomycin (1μM) stimulated TGFβig-h3 secretion comparable to IGF-I (50ng.ml<sup>-1</sup>) and IGF-II (100ng.ml<sup>-1</sup>). <i>D</i>. In calcium-free medium stimulated secretion in response to IGF-II is inhibited.</p>