10.1371/journal.pone.0141331.g001
J. Dinesh Kumar
J. Dinesh
Kumar
Chris Holmberg
Chris
Holmberg
Silvia Balabanova
Silvia
Balabanova
Lyudmyla Borysova
Lyudmyla
Borysova
Ted Burdyga
Ted
Burdyga
Robert Beynon
Robert
Beynon
Graham J. Dockray
Graham J.
Dockray
Andrea Varro
Andrea
Varro
Secretion of TGFβig-h3 is stimulated by chemerin and IGF.
Public Library of Science
2015
TGF βig
adhesion
receptor tyrosine kinase stimulant
exocytosi
igf
Mesenchymal Stem Cells Exhibit Regulated Exocytosis
secretory protein transport
secretion
chemerin
mmp
gene ontology analysis
exhibit
preformed secretory vesicles
msc
2015-10-29 03:25:18
Figure
https://plos.figshare.com/articles/figure/_Secretion_of_TGF_946_ig_h3_is_stimulated_by_chemerin_and_IGF_/1589258
<p><i>A</i>. Western blot analysis of TGFβig-h3 in media from MSC cells shows stimulation by chemerin and IGF-II and inhibition by CCX832 and AG1042, respectively. <i>B</i>. Stimulated secretion of TGFβig-h3 is maintained after cycloheximide treatment. TGFβig-h3 abundance in cell extracts was unchanged (middle panel); GAPDH was used as a loading control for the cell extracts (bottom panel). <i>C</i>. The calcium ionophore, ionomycin (1μM) stimulated TGFβig-h3 secretion comparable to IGF-I (50ng.ml<sup>-1</sup>) and IGF-II (100ng.ml<sup>-1</sup>). <i>D</i>. In calcium-free medium stimulated secretion in response to IGF-II is inhibited.</p>