%0 Figure %A Peng, Yanyan %A Shi, Kaikai %A Wang, Lintao %A Lu, Jianlei %A Li, Hongwei %A Pan, Shiyang %A Ma, Changyan %D 2013 %T Both endogenous and exogenous Osx are increased by proteasome inhibitor treatment. %U https://plos.figshare.com/articles/figure/_Both_endogenous_and_exogenous_Osx_are_increased_by_proteasome_inhibitor_treatment_/158042 %R 10.1371/journal.pone.0056451.g003 %2 https://plos.figshare.com/ndownloader/files/487528 %K endogenous %K exogenous %K osx %K proteasome %K inhibitor %X

A. Saos-2 cells were treated with MG-132 (20 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. B. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with MG-132 (20 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. C. Saos-2 cells were treated with lactacystin (10 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. D. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with lactacystin (10 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. β-actin served as a loading control. Results are shown for one of three independent experiments.

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