Both endogenous and exogenous Osx are increased by proteasome inhibitor treatment.
Yanyan Peng
Kaikai Shi
Lintao Wang
Jianlei Lu
Hongwei Li
Shiyang Pan
Changyan Ma
10.1371/journal.pone.0056451.g003
https://plos.figshare.com/articles/figure/_Both_endogenous_and_exogenous_Osx_are_increased_by_proteasome_inhibitor_treatment_/158042
<p>A. Saos-2 cells were treated with MG-132 (20 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. B. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with MG-132 (20 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. C. Saos-2 cells were treated with lactacystin (10 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. D. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with lactacystin (10 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. β-actin served as a loading control. Results are shown for one of three independent experiments.</p>
2013-02-19 15:15:56
endogenous
exogenous
osx
proteasome
inhibitor