10.1371/journal.pone.0056451.g003
Yanyan Peng
Yanyan
Peng
Kaikai Shi
Kaikai
Shi
Lintao Wang
Lintao
Wang
Jianlei Lu
Jianlei
Lu
Hongwei Li
Hongwei
Li
Shiyang Pan
Shiyang
Pan
Changyan Ma
Changyan
Ma
Both endogenous and exogenous Osx are increased by proteasome inhibitor treatment.
Public Library of Science
2013
endogenous
exogenous
osx
proteasome
inhibitor
2013-02-19 15:15:56
Figure
https://plos.figshare.com/articles/figure/_Both_endogenous_and_exogenous_Osx_are_increased_by_proteasome_inhibitor_treatment_/158042
<p>A. Saos-2 cells were treated with MG-132 (20 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. B. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with MG-132 (20 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. C. Saos-2 cells were treated with lactacystin (10 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. D. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with lactacystin (10 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. β-actin served as a loading control. Results are shown for one of three independent experiments.</p>