10.1371/journal.pone.0056451.g003 Yanyan Peng Yanyan Peng Kaikai Shi Kaikai Shi Lintao Wang Lintao Wang Jianlei Lu Jianlei Lu Hongwei Li Hongwei Li Shiyang Pan Shiyang Pan Changyan Ma Changyan Ma Both endogenous and exogenous Osx are increased by proteasome inhibitor treatment. Public Library of Science 2013 endogenous exogenous osx proteasome inhibitor 2013-02-19 15:15:56 Figure https://plos.figshare.com/articles/figure/_Both_endogenous_and_exogenous_Osx_are_increased_by_proteasome_inhibitor_treatment_/158042 <p>A. Saos-2 cells were treated with MG-132 (20 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. B. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with MG-132 (20 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. C. Saos-2 cells were treated with lactacystin (10 µM) for various periods of time. Endogenous Osx protein was detected by Western blot analysis with an anti-Osx antibody. D. HEK 293T cells were transfected with Flag-hOsx plasmids and then treated with lactacystin (10 µM) for various periods of time. Exogenous Osx protein was detected by Western blot analysis with an anti-Flag antibody. β-actin served as a loading control. Results are shown for one of three independent experiments.</p>