%0 Figure %A Kluba, Malgorzata %A Engelborghs, Yves %A Hofkens, Johan %A Mizuno, Hideaki %D 2015 %T Threonine phosphorylation at JM regions influences the monomer–dimer equilibrium of EGF-bound EGFR. %U https://plos.figshare.com/articles/figure/_Threonine_phosphorylation_at_JM_regions_influences_the_monomer_8211_dimer_equilibrium_of_EGF_bound_EGFR_/1575194 %R 10.1371/journal.pone.0139971.g004 %2 https://plos.figshare.com/ndownloader/files/2359436 %K EGFR dimer formation %K receptor monomerization %K monomeric state %K feedback loop %K pkd %K epidermal growth factor receptor %K protein kinase D %K EGFR Signaling Dimerization %K feedback mechanism %K juxtamembrane threonine residues %K raster image correlation spectroscopy %K Signal transduction %K dimerization state %K oscillatory behavior %K Receptor Dimerization %K 2.5 min %X

(A-D) Time traces of the diffusion coefficient of the EGFR with JM region threonine residue mutations; phosphodeficient mutant, EGFRT654/669A (A), three phosphomimic mutants, EGFRT654/669E (B), EGFRT654E (C), and EGFRT669E (D). (E) EGF (Alexa647-labeled, 0.17 μM; magenta) binding to EGFRT654/669E (green) expressing cell. The right panel shows the merged image. Scale bars represent 10 μm.

%I PLOS ONE