Participation of nPKC and PKD1 in the feedback monomerization of the EGFR. Malgorzata Kluba Yves Engelborghs Johan Hofkens Hideaki Mizuno 10.1371/journal.pone.0139971.g003 https://plos.figshare.com/articles/figure/_Participation_of_nPKC_and_PKD1_in_the_feedback_monomerization_of_the_EGFR_/1575193 <p>(A) Time traces of diffusion coefficient of EGFR<sup>wt</sup> in the presence of the PKC activator PMA (upper) or under nominally Ca<sup>2+</sup>-free conditions (lower panel). In red, the time trace of cytosolic Ca<sup>2+</sup> concentration monitored with Fura Red is shown. (B) Time traces of diffusion coefficient of eGFP-EGFR<sup>wt</sup> in the cell coexpressing constitutively active mutant, mCherry-PKD1<sup>S738/742E</sup> (upper), in the cell pre-treated with 10 μM CID755673, PKD1 inhibitor (middle), or in the cell coexpressing kinase deficient mutant, mCherry-PKD1<sup>K612W</sup> (lower panel).</p> 2015-10-14 04:12:03 EGFR dimer formation receptor monomerization monomeric state feedback loop pkd epidermal growth factor receptor protein kinase D EGFR Signaling Dimerization feedback mechanism juxtamembrane threonine residues raster image correlation spectroscopy Signal transduction dimerization state oscillatory behavior Receptor Dimerization 2.5 min