10.1371/journal.pone.0139971.g002
Malgorzata Kluba
Malgorzata
Kluba
Yves Engelborghs
Yves
Engelborghs
Johan Hofkens
Johan
Hofkens
Hideaki Mizuno
Hideaki
Mizuno
Participation of PLCγ1 in the feedback monomerization of the EGFR.
Public Library of Science
2015
EGFR dimer formation
receptor monomerization
monomeric state
feedback loop
pkd
epidermal growth factor receptor
protein kinase D
EGFR Signaling Dimerization
feedback mechanism
juxtamembrane threonine residues
raster image correlation spectroscopy
Signal transduction
dimerization state
oscillatory behavior
Receptor Dimerization
2.5 min
2015-10-14 04:12:03
Figure
https://plos.figshare.com/articles/figure/_Participation_of_PLC_947_1_in_the_feedback_monomerization_of_the_EGFR_/1575192
<p>(A) ccRICS analysis of PLCγ1 binding to EGFR upon EGF challenge. 2D cross-correlation of eGFP-EGFR<sup>wt</sup> and mCherry-PLCγ1<sup>wt</sup> in CHO-K1 cells before (left) and after (right) EGF addition. (B) Cross-correlation index (CC<sub>i</sub>) between mCherry-PLCγ1, eGFP-EGFR, and their mutants before (white) and after (grey) EGF addition. Lyn-mCherry + eGFP (1) and lyn-mCherry + lyn-eGFP (2) were used as negative controls (CTRL). Data are means ± SD. Symbol * indicates significant difference with p < 0.005 calculated using a two sided T-test with unequal variance. (C) Time traces of diffusion coefficient of eGFP-EGFR<sup>Y992/1148/1173F</sup>, lacking PLCγ binding sites in the upper panel, and eGFP-EGFR<sup>wt</sup> coexpressed with mCherry-PLCγ1<sup>Y783F</sup> (inactive mutant, lower panel).</p>