10.1371/journal.pone.0139971.g002 Malgorzata Kluba Malgorzata Kluba Yves Engelborghs Yves Engelborghs Johan Hofkens Johan Hofkens Hideaki Mizuno Hideaki Mizuno Participation of PLCγ1 in the feedback monomerization of the EGFR. Public Library of Science 2015 EGFR dimer formation receptor monomerization monomeric state feedback loop pkd epidermal growth factor receptor protein kinase D EGFR Signaling Dimerization feedback mechanism juxtamembrane threonine residues raster image correlation spectroscopy Signal transduction dimerization state oscillatory behavior Receptor Dimerization 2.5 min 2015-10-14 04:12:03 Figure https://plos.figshare.com/articles/figure/_Participation_of_PLC_947_1_in_the_feedback_monomerization_of_the_EGFR_/1575192 <p>(A) ccRICS analysis of PLCγ1 binding to EGFR upon EGF challenge. 2D cross-correlation of eGFP-EGFR<sup>wt</sup> and mCherry-PLCγ1<sup>wt</sup> in CHO-K1 cells before (left) and after (right) EGF addition. (B) Cross-correlation index (CC<sub>i</sub>) between mCherry-PLCγ1, eGFP-EGFR, and their mutants before (white) and after (grey) EGF addition. Lyn-mCherry + eGFP (1) and lyn-mCherry + lyn-eGFP (2) were used as negative controls (CTRL). Data are means ± SD. Symbol * indicates significant difference with p < 0.005 calculated using a two sided T-test with unequal variance. (C) Time traces of diffusion coefficient of eGFP-EGFR<sup>Y992/1148/1173F</sup>, lacking PLCγ binding sites in the upper panel, and eGFP-EGFR<sup>wt</sup> coexpressed with mCherry-PLCγ1<sup>Y783F</sup> (inactive mutant, lower panel).</p>