%0 Figure %A Glover, Kyle P. %A Chen, Zhongqiang %A K. Markell, Lauren %A Han, Xing %D 2015 %T Validation of seventeen gene signature with other PKC-activating tumor promoters. %U https://plos.figshare.com/articles/figure/_Validation_of_seventeen_gene_signature_with_other_PKC_activating_tumor_promoters_/1562988 %R 10.1371/journal.pone.0139850.g009 %2 https://plos.figshare.com/ndownloader/files/2297156 %K TK 6 cells %K ROPA %K tpa %K Human lymphoblastoid TK 6 cells %K uvc %K pcr %K protein kinase C %K 17 gene signature %K ap %K gene expression patterns %K Synergistic Gene Expression Signature Observed %K dna %K stress response pathways %K novel gene signature %K tumor microenvironment %K qpcr %K gene expression signatures %X

TK6 cells were treated with other PKC-activating tumor promoters including PDBu, SPd, Mez, IndV and ROPA and exposed to UVC-irradiation to verify the 17 gene signature observed with RNA-sequencing. Gene expression was observed with QPCR. Most of the 17 genes had synergistic expression patterns in the co-treated cells (green bar). The fold-change of the UVC-alone (grey bar) and PKC-activator alone (red bar) are stacked to represent the theoretical additive response. One way ANOVA with Bonferroni post-hoc analysis was conducted (p<0.05) to determine statistical significance of the co-treated cells compared to either stress alone.

%I PLOS ONE