10.1371/journal.pone.0139072.g002
Thomas Westergard
Thomas
Westergard
Reza Salari
Reza
Salari
Joseph V. Martin
Joseph
V. Martin
Grace Brannigan
Grace
Brannigan
Inhibition of GABA response by T3.
Public Library of Science
2015
T 3 molecules
dynamic
ALLOP binding site
hormone
M 1 backbone
T 3
Overlapping Intersubunit Binding Modes Structural mechanisms
md
ivm
simulation
intersubunit sites
gaba
subunit interfaces
receptor
neurosteroid
gabaa
2015-09-30 03:05:14
Figure
https://plos.figshare.com/articles/figure/_Inhibition_of_GABA_response_by_T3_/1560372
<p>(A) Dose-response curve for the effects of T3 on the GABA-stimulated current as a percent of the maximal GABA response in the absence of T3. The values are expressed as a mean of three separate determinations, with error bars representing the standard error of the mean (S.E.M.). (A, Inset) Representative tracings for the effect of 10 μM GABA with or without added 10 μM T3. The solid lines above the tracing indicate the time of superfusion of the oocyte with the indicated solutions. (B) Evaluation of T3 inhibition of GABA response. Dose-response curves for the effects of GABA were constructed separately in the presence of 0, 5, 10, or 20 μM T3. The data are represented as means ± S.E.M. for triplicate determinations. For each data point, <i>n</i> = 3–5. (B, Inset) Schild plot of the data from (B). “Dr” stands for dose-ratio. The slope of the line was 0.04 ± 0.02, which was significantly different from unity according to 95% confidence levels (shown in dotted lines).</p>