10.1371/journal.pone.0138828.g001 Nadège Philippe Nadège Philippe Laure Maigre Laure Maigre Sébastien Santini Sébastien Santini Elizabeth Pinet Elizabeth Pinet Jean-Michel Claverie Jean-Michel Claverie Anne-Véronique Davin-Régli Anne-Véronique Davin-Régli Jean-Marie Pagès Jean-Marie Pagès Muriel Masi Muriel Masi Hoechst 33342 influx into <i>E</i>. <i>aerogenes</i> strains. Public Library of Science 2015 encoding sensor kinases membrane permeability nosocomial pathogen control membrane permeability Enterobacter aerogenes Infections Bacterial Resistance antimicrobial strategies transport assays antibiotic mdr omp 35 Enterobacter aerogenes membrane permeability defects Imipenem Infections membrane alterations link membrane permeability vivo evolution G 7 efflux pumps 2015-09-23 03:54:05 Figure https://plos.figshare.com/articles/figure/_Hoechst_33342_influx_into_E_aerogenes_strains_/1554199 <p>Strains were grown in LB broth and cells were harvested at the beginning of the stationary phase. Cells were washed, resuspended in PPB pH 7.0, and the H33342 influx was assayed in the presence of 50 μM CCCP. A. Chemical structure of H33342. B. Kinetics of H33342 influx. AU, arbitrary units. C. Total accumulation in <i>E</i>. <i>aerogenes</i> isolates after normalization to that of strain ATCC15038. All values are means of at least three independent experiments.</p>