10.1371/journal.pgen.1005505.g004 Trevor L. Cameron Trevor L. Cameron Katrina M. Bell Katrina M. Bell Irma L. Gresshoff Irma L. Gresshoff Lisa Sampurno Lisa Sampurno Lorna Mullan Lorna Mullan Joerg Ermann Joerg Ermann Laurie H. Glimcher Laurie H. Glimcher Raymond P. Boot-Handford Raymond P. Boot-Handford John F. Bateman John F. Bateman Quantitative PCR of mutant and wildtype hypertrophic zones. Public Library of Science 2015 Disease Schmid metaphyseal chondrodysplasia canonical ER stress sensors XBP exogenous misfolding protein chop perk hypertrophic zone collagen GADD chondrocyte differentiation hypertrophic chondrocytes upr atf Col 10a Mouse models phenocopying MCDS pathology ColXN 617K growth plate cartilage hypertrophic zone expansion hypertrophic zone cartilage hypertrophic zone expansion ire runx 2015-09-15 03:14:21 Figure https://plos.figshare.com/articles/figure/_Quantitative_PCR_of_mutant_and_wildtype_hypertrophic_zones_/1545161 <p>qPCR with primers specific for <i>(A) Agc1</i>, <i>(B) Ctgf</i>, <i>(C) Matn1</i>, <i>(D) Creld2</i>, <i>(E) Derl3</i>, <i>(F) Ero1l</i>, <i>(G) Fgf21</i>, <i>(H) Steap1</i>, and <i>(I) p58IPK</i> on cDNA derived from <i>Wt</i>, <i>Xbp1</i><sup><i>CartΔEx2</i></sup>, <i>ColX</i><sup><i>N617K</i></sup> and <i>C/X</i> hypertrophic zone aRNA. Plots depict mean fold differences with standard deviation from the mean, N = 3, statistical significance was determined using Student’s <i>t</i> test, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001, **** <i>p</i> < 0.0001.</p>