%0 Figure %A Iscla, Irene %A Wray, Robin %A Eaton, Christina %A Blount, Paul %D 2015 %T Effects of post translational modifications on the periplasmic loop of MscL determined by in vivo channel activity. %U https://plos.figshare.com/articles/figure/_Effects_of_post_translational_modifications_on_the_periplasmic_loop_of_MscL_determined_by_in_vivo_channel_activity_/1543025 %R 10.1371/journal.pone.0137994.g006 %2 https://plos.figshare.com/ndownloader/files/2263349 %K Scanning MscL Channels %K channel function %K 113 MscL cysteine mutants %K emergency release valve %K protein %K encode mechanosensitive channel activity %K Functional Alterations Mechanosensitive channels %X

The viability of the osmotically fragile strain MJF455 expressing MscL cysteine substituted mutants from residues L47 to H74 (insert), was measured after an osmotic down-shock. The graphs show the differences in viability between non-treated and post-transnationally modified channels with (A) the hydrophobic MTS reagents MTSBn (blue), decyl-MTS (red) or 4HB-MTS (green) or (B) the negatively charged MTSES- (blue) and positively charged MTSET+ (red). The red grid line indicates a ± 50% change that was used as a threshold for further studies.

%I PLOS ONE