Ishibashi, Daisuke Homma, Takujiro Nakagaki, Takehiro Fuse, Takayuki Sano, Kazunori Takatsuki, Hanae Atarashi, Ryuichiro Nishida, Noriyuki PrP<sup>Sc</sup> in N2a-FK cells undergoes degradation via upstream intracellular signalling cascades associated with autophagy. <p>(A) N2a-FK cells were treated with 0.1 to 10 μM of the the PI3K inhibitor LY294002 and 1 to 50 μM of the MEK inhibitor of PD98059 for 48 h. PK-treated or-untreated samples were applied at concentrations of 100 and 50 μg protein per lane onto a 15% polyacrylamide gel and subjected to SDS-PAGE. The proteins were analyzed by western blotting using anti-PrP, anti-Akt, anti-phosphorylated Akt (to determine the Akt activation level), anti-p44/p42 MAPK, anti-phosphorylated p44/p42 MAPK (to determine the p44/p42 MAPK activation level) and anti-β-actin antibodies. (B) The effect of these drugs on PrP<sup>Sc</sup> was determined by quantifying the PrP<sup>Sc</sup> band intensities as a percentage of those of the negative controls. The results in the graph are the mean ± SD of at least three independent experiments. *p < 0.05 and **p < 0.01 (one-way ANOVA followed by Tukey's test).</p> macroautophagy inducer rapamycin;fk;cells stably;Prion Strains;Chandler strain;22 L;Infected Neuronal Cells Prion diseases;Host cells;neurodegenerative disorders;signal transduction pathways;PrPSc degradation;Abnormally Folded Prion Protein Degradation;3 MA;prion protein;prion strain Fukuoka;accumulation 2015-09-14
    https://plos.figshare.com/articles/figure/_PrP_Sc_in_N2a_FK_cells_undergoes_degradation_via_upstream_intracellular_signalling_cascades_associated_with_autophagy_/1541295
10.1371/journal.pone.0137958.g004