Li, Xinlu Ménade, Marie Kozlov, Guennadi Hu, Zheping Dai, Zheng S. McPherson, Peter Brais, Bernard Gehring, Kalle Confirmation of HTS candidates by FP competition assay. <p>A and B. 5 nM F12-ATP and 200 nM GST-HEPN were titrated with the hits to confirm their binding to GST-HEPN. C. Affinities of the compounds for HEPN were estimated from the competition assays and summarized along with their chemical structures.</p> cell culture studies;neuron;binding;neurodegenerative disease autosomal recessive spastic ataxia;ARSACS;sacsin;protein quality control;nucleotide;HEPN domain;FP competition assays 2015-09-14
    https://plos.figshare.com/articles/figure/_Confirmation_of_HTS_candidates_by_FP_competition_assay_/1541283
10.1371/journal.pone.0137298.g005