Confirmation of HTS candidates by FP competition assay. Xinlu Li Marie Ménade Guennadi Kozlov Zheping Hu Zheng Dai Peter S. McPherson Bernard Brais Kalle Gehring 10.1371/journal.pone.0137298.g005 https://plos.figshare.com/articles/figure/_Confirmation_of_HTS_candidates_by_FP_competition_assay_/1541283 <p>A and B. 5 nM F12-ATP and 200 nM GST-HEPN were titrated with the hits to confirm their binding to GST-HEPN. C. Affinities of the compounds for HEPN were estimated from the competition assays and summarized along with their chemical structures.</p> 2015-09-14 02:46:08 cell culture studies neuron binding neurodegenerative disease autosomal recessive spastic ataxia ARSACS sacsin protein quality control nucleotide HEPN domain FP competition assays