10.1371/journal.pone.0137508.g005 Viveka Vadyvaloo Viveka Vadyvaloo Angela K. Hinz Angela K. Hinz RovM senses nutritional cues. Public Library of Science 2015 biofilm gut blockage kim rovm transcriptional levels flea gut environment fitness defect type Senses Nutritional Cues Suggesting infection strain Flea Gut Yersinia pestis flea gut colonization Δ rovM 2015-09-08 03:16:18 Figure https://plos.figshare.com/articles/figure/_RovM_senses_nutritional_cues_/1537196 <p>Growth of WT (pACYC177) (●), Δ<i>rovM</i> (pACYC177) mutant (■) and Δ<i>rovM</i> (pACrovM) complemented mutant (♦) strains in Sebbane minimal medium (SMM) supplemented with 0.2% (A) glucose (dark grey), galactose (light grey), or diacteylchitobiose (black) as additional carbon sources or, (B) amino acids, glutamine (light grey), arginine (dark grey) and histidine (black). Error bars indicate the mean±SD of three independent experiments. To calculate the growth rate linear regression analysis was performed on the exponential phase time points of growth between 18–32 hours for (A) and 16–32 hours for (B). The <i>ΔrovM</i> (pACrovM) complemented strain grew at a significantly higher growth rate (*, P<0.001) in SMM supplemented with diacetylchitobiose, glutamine and arginine compared to the Δ<i>rovM</i> mutant and wild type strains. WT = wild type.</p>