Functional expression of concatenated ASIC1a fusion proteins.
Miguel Xavier van Bemmelen
Delphine Huser
Ivan Gautschi
Laurent Schild
10.1371/journal.pone.0135191.g005
https://plos.figshare.com/articles/figure/_Functional_expression_of_concatenated_ASIC1a_fusion_proteins_/1506179
<p>A: Representative recording of an ASIC1a current in Xenopus oocytes expressing a fusion protein made of three ASIC1a subunit proteins, each with intact C-termini, and linked in a head to tail fashion (3xFP). B: Average (mean ± SD) of ASIC1a currents elicited at pH 6.0 measured in Xenopus oocytes expressing monomeric hASIC1a (wt or G433C mutant) or fusion proteins made of two (2xFP), three (3xFP), or four (4xFP) ASIC1a subunit proteins. All constructs comprise a single, N-terminal, His<sub>8</sub> tag. C: Anti-ASIC1a western blot obtained from oocytes expressing the same ASIC1a constructs as in B; I, II, III, IV have the same meaning as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135191#pone.0135191.g003" target="_blank">Fig 3A</a>. D: Relationship between the molecular weights of bands I, II, III, IV estimated from ASIC1a oligomers crosslinked with BMOE (as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135191#pone.0135191.g003" target="_blank">Fig 3A</a>) and the size (kDa) of dimeric (gray), trimeric (red) and tetrameric (blue) ASIC1a fusion proteins; the straight dotted line represents the linear regression fit forced to the axes origin; the slope of the regression line is 0.993 ± 0.052.</p>
2015-08-07 04:03:38
extracellular pore vestibule
oligomeric states
ASIC 1a complexes
Xenopus laevis oocytes
sulfhydryl crosslinker BMOE
Homotetrameric Assembly State
ASIC 1a monomers
crosslinking conditions ASIC 1a
ASIC 1a oligomers
ASIC 1a subunit
cell surface
ASIC 1a channel
tetrameric concatemeric cDNA
cho
ASIC 1a homotetramer
ASIC 1 complexes