Functional expression of concatenated ASIC1a fusion proteins. Miguel Xavier van Bemmelen Delphine Huser Ivan Gautschi Laurent Schild 10.1371/journal.pone.0135191.g005 https://plos.figshare.com/articles/figure/_Functional_expression_of_concatenated_ASIC1a_fusion_proteins_/1506179 <p>A: Representative recording of an ASIC1a current in Xenopus oocytes expressing a fusion protein made of three ASIC1a subunit proteins, each with intact C-termini, and linked in a head to tail fashion (3xFP). B: Average (mean ± SD) of ASIC1a currents elicited at pH 6.0 measured in Xenopus oocytes expressing monomeric hASIC1a (wt or G433C mutant) or fusion proteins made of two (2xFP), three (3xFP), or four (4xFP) ASIC1a subunit proteins. All constructs comprise a single, N-terminal, His<sub>8</sub> tag. C: Anti-ASIC1a western blot obtained from oocytes expressing the same ASIC1a constructs as in B; I, II, III, IV have the same meaning as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135191#pone.0135191.g003" target="_blank">Fig 3A</a>. D: Relationship between the molecular weights of bands I, II, III, IV estimated from ASIC1a oligomers crosslinked with BMOE (as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135191#pone.0135191.g003" target="_blank">Fig 3A</a>) and the size (kDa) of dimeric (gray), trimeric (red) and tetrameric (blue) ASIC1a fusion proteins; the straight dotted line represents the linear regression fit forced to the axes origin; the slope of the regression line is 0.993 ± 0.052.</p> 2015-08-07 04:03:38 extracellular pore vestibule oligomeric states ASIC 1a complexes Xenopus laevis oocytes sulfhydryl crosslinker BMOE Homotetrameric Assembly State ASIC 1a monomers crosslinking conditions ASIC 1a ASIC 1a oligomers ASIC 1a subunit cell surface ASIC 1a channel tetrameric concatemeric cDNA cho ASIC 1a homotetramer ASIC 1 complexes