10.1371/journal.pone.0133770.g001 Xuewen Jin Xuewen Jin Yuling Liu Yuling Liu Jingjing Liu Jingjing Liu Weiliang Lu Weiliang Lu Ziwei Liang Ziwei Liang Dan Zhang Dan Zhang Gang Liu Gang Liu Hongxia Zhu Hongxia Zhu Ningzhi Xu Ningzhi Xu Shufang Liang Shufang Liang IQGAP1 interacts with β-catenin and regulates β-catenin expression. Public Library of Science 2015 Cell proliferation catenin IQGAP 1 multifunctional scaffold protein IQGAP 1 cooperation cell metastasis ability HCC cell lines migration ability expression rna 2015-08-07 04:02:18 Figure https://plos.figshare.com/articles/figure/_IQGAP1_interacts_with_946_catenin_and_regulates_946_catenin_expression_/1506173 <p>(A) Endogenous IQGAP1 interacts with β-catenin in HepG2 cells. 500ug of HepG2 cell lysate was immunoprecipitated with anti-IQGAP1 antibodies, and bound proteins were analyzed by immunoblotting with the indicated antibodies. 5% of total lysate was loaded as a control (input). (B) The IQGAP1 overexpression improved the expression of β-catenin. Transfection with pCMV6 empty plasmids was taken as a control and the untreated HepG2 cells were taken as a mock. flag-IQ: pFlag-IQGAP1 plasmids. (C) The IQGAP1 knockdown by IQGAP1 siRNA (si-IQ) reduced β-catenin expression. Transfection with non-target siRNA was taken as a control (si-NC), and untreated HepG2 cells were taken as a mock. (D)The expression of IQGAP1 and β-catenin was gradually increased in several HCC cell lines. LO2 was a normal liver cell line. 97L: MHCC-97L; 97H: MHCC-97H.</p>