10.1371/journal.pone.0133770.g001
Xuewen Jin
Xuewen
Jin
Yuling Liu
Yuling
Liu
Jingjing Liu
Jingjing
Liu
Weiliang Lu
Weiliang
Lu
Ziwei Liang
Ziwei
Liang
Dan Zhang
Dan
Zhang
Gang Liu
Gang
Liu
Hongxia Zhu
Hongxia
Zhu
Ningzhi Xu
Ningzhi
Xu
Shufang Liang
Shufang
Liang
IQGAP1 interacts with β-catenin and regulates β-catenin expression.
Public Library of Science
2015
Cell proliferation
catenin
IQGAP 1
multifunctional scaffold protein
IQGAP 1 cooperation
cell metastasis ability
HCC cell lines
migration ability
expression
rna
2015-08-07 04:02:18
Figure
https://plos.figshare.com/articles/figure/_IQGAP1_interacts_with_946_catenin_and_regulates_946_catenin_expression_/1506173
<p>(A) Endogenous IQGAP1 interacts with β-catenin in HepG2 cells. 500ug of HepG2 cell lysate was immunoprecipitated with anti-IQGAP1 antibodies, and bound proteins were analyzed by immunoblotting with the indicated antibodies. 5% of total lysate was loaded as a control (input). (B) The IQGAP1 overexpression improved the expression of β-catenin. Transfection with pCMV6 empty plasmids was taken as a control and the untreated HepG2 cells were taken as a mock. flag-IQ: pFlag-IQGAP1 plasmids. (C) The IQGAP1 knockdown by IQGAP1 siRNA (si-IQ) reduced β-catenin expression. Transfection with non-target siRNA was taken as a control (si-NC), and untreated HepG2 cells were taken as a mock. (D)The expression of IQGAP1 and β-catenin was gradually increased in several HCC cell lines. LO2 was a normal liver cell line. 97L: MHCC-97L; 97H: MHCC-97H.</p>