%0 Figure %A Stamatakou, Eleanna %A Hoyos-Flight, Monica %A C. Salinas, Patricia %D 2015 %T Eps8 is required for Wnt3a-mediated axonal remodeling. %U https://plos.figshare.com/articles/figure/_Eps8_is_required_for_Wnt3a_mediated_axonal_remodeling_/1506100 %R 10.1371/journal.pone.0134976.g006 %2 https://plos.figshare.com/ndownloader/files/2205939 %K abolishes Wnt 3a axonal remodelling %K axon remodelling activity %K Wnt 3a increases actin dynamics %K Wnt 3a increases lamellar protrusion %K dorsal root ganglia neurons %K Wnt Signalling Promotes Actin Dynamics %K Wnt 3a Importantly %K axonal remodelling %K nt %K rodent DRG neurons %K Eps 8 %K scaffold protein Dishevelled %K actin cytoskeleton %K growth cones %X

(A) Eps8 promotes growth cone enlargement and increases F-actin accumulation in growth cones. Pseudocolor images show the different levels of F-actin in growth cones of Eps8-expressing and control GFP-expressing cells. Scale bar: 5μm. (B and C) Growth cone area is larger in Eps8-expressing cells than in controls cells, as is the percentage of growth cone area with high F-actin fluorescence. (D) DRG neurons expressing scramble or Eps8 shRNAs were treated with Wnt3a for 2 hrs. Scale bar: 5μm. (E) In scrambled shRNA-expressing cells Wnt3a increases the percentage of cells that show axonal remodelling (% of cells remodelled) and (F) promotes growth cone enlargement. In contrast, neurons expressing Eps8shRNAs do not respond to Wnt3a. *p<0.05, **p<0.01, ***p<0.001.

%I PLOS ONE