Presence of PrtV in OMVs from <i>V</i>. <i>cholerae</i> strain C6706.
Pramod K. Rompikuntal
Svitlana Vdovikova
Marylise Duperthuy
Tanya L. Johnson
Monika Ã…hlund
Richard Lundmark
Jan Oscarsson
Maria Sandkvist
Bernt Eric Uhlin
Sun Nyunt Wai
10.1371/journal.pone.0134098.g002
https://plos.figshare.com/articles/figure/_Presence_of_PrtV_in_OMVs_from_V_cholerae_strain_C6706_/1496986
<p>To detect the PrtV and the OmpU proteins in the density gradient fractions, immunoblot analyses were performed using polyclonal anti-PrtV and anti-OmpU antisera, respectively. (A) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the wild type <i>V</i>. <i>cholerae</i> strain C6706. (B) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the <i>prtV</i> mutant. (C) Immunobot detection of PrtV in the whole cell lysate (lane 1), culture supernatant before ultracentrifugation (lane 2), supernatant after the removal of OMVs (lane 3), and OMV sample (lane 4).</p>
2015-07-29 03:29:09
protease
cholerae strain C 6706
type II secretion system
Vibrio cholerae BackgroundOuter membrane vesicles
hct
type V
Processed PrtV Protease
Target Host Cells
omv
ll