Presence of PrtV in OMVs from <i>V</i>. <i>cholerae</i> strain C6706. Pramod K. Rompikuntal Svitlana Vdovikova Marylise Duperthuy Tanya L. Johnson Monika Ã…hlund Richard Lundmark Jan Oscarsson Maria Sandkvist Bernt Eric Uhlin Sun Nyunt Wai 10.1371/journal.pone.0134098.g002 https://plos.figshare.com/articles/figure/_Presence_of_PrtV_in_OMVs_from_V_cholerae_strain_C6706_/1496986 <p>To detect the PrtV and the OmpU proteins in the density gradient fractions, immunoblot analyses were performed using polyclonal anti-PrtV and anti-OmpU antisera, respectively. (A) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the wild type <i>V</i>. <i>cholerae</i> strain C6706. (B) Immunoblot detection of PrtV (upper panel) and OmpU (lower panel) in density gradient fractions of OMVs from the <i>prtV</i> mutant. (C) Immunobot detection of PrtV in the whole cell lysate (lane 1), culture supernatant before ultracentrifugation (lane 2), supernatant after the removal of OMVs (lane 3), and OMV sample (lane 4).</p> 2015-07-29 03:29:09 protease cholerae strain C 6706 type II secretion system Vibrio cholerae BackgroundOuter membrane vesicles hct type V Processed PrtV Protease Target Host Cells omv ll