10.1371/journal.pone.0128916.g001 Jonathan A. Scolnick Jonathan A. Scolnick Michelle Dimon Michelle Dimon I-Ching Wang I-Ching Wang Stephanie C. Huelga Stephanie C. Huelga Douglas A. Amorese Douglas A. Amorese Description of the Ovation Target Enrichment System. Public Library of Science 2015 RNA sequencing fusion gene identification Targeted RNA Sequencing fusion genes cancer fusion genes gene fusions FFPE Samples Fusion genes Single Primer Enrichment Technology SPET target 5701 exons 2015-07-01 02:46:07 Figure https://plos.figshare.com/articles/figure/_Description_of_the_Ovation_Target_Enrichment_System_/1470183 <p>(A) Experimental steps of the assay and time required for each step. Adaptors (green) are ligated on to generated double stranded cDNA (ds-cDNA). Probes (shown in red and yellow) are hybridized to target cDNA and extended with a polymerase (dashed grey lines). All probes have common tail sequences (blue), which are used as priming sites along with adaptor sequences in subsequent library amplification PCR steps. (B) Example of probe positioning across different exons in a full length double stranded cDNA. Each exon (demarked by blue vertical lines) will have probes (green arrows; arrow points in the 3’ direction) designed to hybridize near the predicted exon-exon junctions. Exons larger than 300 nucleotides (nt) may have additional probes tiled along the length of the exon to obtain more complete sequence coverage. Probes are designed against both strands of the cDNA to enable identification of gene fusions when only one of the pair of genes is targeted. Translation start sequence (ATG) and poly A tail are labeled.</p>