Double staining of a larval wing imaginal disk and pupal wing tissue. OhnoYoshikazu OtakiJoji M. 2015 <p>The tissue was stained with SYBR Green I for nuclei and MitoTacker Orange for mitochondria. Scale bars indicate 10 μm. (<b>a</b>) Larval wing imaginal disk. A portion is enlarged in the right panel. An optical vertical cross-section image shown at the bottom was made along a blue broken line. Thickness of a cell was approximately 2.9 μm. The image was constructed by stacking 28 images down to 5.5 μm in depth with 0.2 μm intervals. (<b>b</b>) Pupal wing tissue. The pupal head is positioned to the left. A portion is enlarged in the right panel. An optical vertical cross-section image shown at the bottom was made along a blue broken line. Thickness of a cell was more than 11.4 μm (the deepest portion not detected). The image was constructed by stacking 58 images with 0.2 μm intervals.</p>