%0 Figure %A Kiebala, Michelle %A V. Singh, Meera %A S. Piepenbrink, Michael %A Qiu, Xing %A J. Kobie, James %A B. Maggirwar, Sanjay %D 2015 %T Cocaine and HIV do not activate platelets in whole blood when treated ex vivo. %U https://plos.figshare.com/articles/figure/_Cocaine_and_HIV_do_not_activate_platelets_in_whole_blood_when_treated_ex_vivo_/1449415 %R 10.1371/journal.pone.0130061.g002 %2 https://plos.figshare.com/ndownloader/files/2113014 %K HIV patients %K HIV infection warrants %K anucleate blood cells %K IKK β. Platelet activation %K kappa B kinase %K nf %K Platelet activation %K CD 62P expression %K cocaine abuse %K Human immunodeficiency virus type %X

Whole blood from healthy subjects (3 to 4 donors) was left untreated (NT) or was treated with cocaine (A), infectious NL4-3 HIV (B), ADP or Tat (C) (D), for 30, 60, and 30 minutes respectively, or as indicated, following which CD62P expression was assessed as a marker of platelet activation via flow cytometry. Treatment with cocaine, HIV, and Tat did not result in increased platelet activation. In each experiment, as indicated, treatment with 1 μL dH2O for 30 minutes was used as a vehicle (Veh) control. Data are represented as fold change in CD62P MFI as compared to NT samples and are shown as mean ± SEM. * p<0.05, ** p<0.01, *** p<0.001.

%I PLOS ONE