10.1371/journal.pone.0128546.g003 Jiro Ikeda Jiro Ikeda Toshihiro Ichiki Toshihiro Ichiki Yusuke Takahara Yusuke Takahara Hiroshi Kojima Hiroshi Kojima Chikahiro Sankoda Chikahiro Sankoda Shiro Kitamoto Shiro Kitamoto Tomotake Tokunou Tomotake Tokunou Kenji Sunagawa Kenji Sunagawa Reversion of the anti-ER stress and anti-apoptotic effects of pioglitazone by GW9662. Public Library of Science 2015 ER stress chop perk raw jnk Western Blot analysis scd type 2 diabetes agonist expression murine macrophage cell line ppar gw Macrophages BackgroundClinical trials ER stress marker 2015-06-10 03:21:56 Figure https://plos.figshare.com/articles/figure/_Reversion_of_the_anti_ER_stress_and_anti_apoptotic_effects_of_pioglitazone_by_GW9662_/1444927 <p>RAW264.7 cells were pretreated with GW9662 (1, 5, 10 μmol/L) for 1 hour and incubated with pioglitazone (10 μmol/L) for 6 hours. Then, cells were stimulated with palmitate (400 μmol/L) for 16 hours. Western blot analyses for phospho (P)-PERK (A), CHOP (B), phospho (P)-JNK (C), cleaved caspase-3 (D) were performed. The bar graphs indicate the ratio of P-PERK to PERK, CHOP to α-tubulin, P-JNK to JNK, and cleaved caspase-3 to α-tubulin, respectively. *P<0.05, ** P<0.01 vs control (palmitate (-), Pioglitazone (-) and GW9662 (-)), #P<0.05 vs palmitate, †P<0.05, ††P<0.01 vs Palmitate+Pioglitazone. n = 4.</p>