Omori, Yoshihiro Chaya, Taro Yoshida, Satoyo Irie, Shoichi Tsujii, Toshinori Furukawa, Takahisa Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control <div><p>Primary cilia are sensory organelles that harbor various receptors such as G protein-coupled receptors (GPCRs). We analyzed subcellular localization of 138 non-odorant GPCRs. We transfected GPCR expression vectors into NIH3T3 cells, induced ciliogenesis by serum starvation, and observed subcellular localization of GPCRs by immunofluorescent staining. We found that several GPCRs whose ligands are involved in feeding behavior, including prolactin-releasing hormone receptor (PRLHR), neuropeptide FF receptor 1 (NPFFR1), and neuromedin U receptor 1 (NMUR1), localized to the primary cilia. In addition, we found that a short form of dopamine receptor D2 (DRD2S) is efficiently transported to the primary cilia, while a long form of dopamine receptor D2 (DRD2L) is rarely transported to the primary cilia. Using an anti-Prlhr antibody, we found that Prlhr localized to the cilia on the surface of the third ventricle in the vicinity of the hypothalamic periventricular nucleus. We generated the <i>Npy2r-Cre</i> transgenic mouse line in which Cre-recombinase is expressed under the control of the promoter of <i>Npy2r</i> encoding a ciliary GPCR. By mating <i>Npy2r-Cre</i> mice with <i>Ift80</i> flox mice, we generated <i>Ift80</i> conditional knockout (CKO) mice in which Npy2r-positive cilia were diminished in number. We found that <i>Ift80</i> CKO mice exhibited a body weight increase. Our results suggest that Npy2r-positive cilia are important for body weight control.</p></div> NPFFR;drd;Subcellular localization;body weight increase;2l;NMUR;Ift 80 CKO mice;mating Npy 2r mice;body weight control;Npy 2r encoding;transfected GPCR expression vectors;Ift 80 flox mice;ff;NIH 3T cells;Npy 2r cilia;neuromedin U receptor 1;dopamine receptor D 2;2s;PRLHR 2015-06-08
    https://plos.figshare.com/articles/dataset/_Identification_of_G_Protein_Coupled_Receptors_GPCRs_in_Primary_Cilia_and_Their_Possible_Involvement_in_Body_Weight_Control_/1440189
10.1371/journal.pone.0128422