Prlhr localizes to cilia in the hypothalamic third ventricle.
Yoshihiro Omori
Taro Chaya
Satoyo Yoshida
Shoichi Irie
Toshinori Tsujii
Takahisa Furukawa
10.1371/journal.pone.0128422.g004
https://plos.figshare.com/articles/figure/_Prlhr_localizes_to_cilia_in_the_hypothalamic_third_ventricle_/1440182
<p><b>A–D</b>) Prlhr was localized to the ventricular cilium in the adult mouse brain. Frozen sections containing the third ventricle of the adult mouse brain were immunostained with an anti-Prlhr antibody (green). The Prlhr signal was observed in cilia protruding from cells on the surface of the third ventricle (arrowheads in A, B, D). Preabsorption of the anti-Prlhr antibody with a synthetic Prlhr-peptide antigen abolished the Prlhr staining in the cilia (C). Cilia were coimmunostained with an anti-Arl13b antibody (D, red). Cilia with the Prlhr signal (arrowheads) were not clustered together, unlike the multiple cilia of ependymal cells that lacked the Prlhr signal (arrows). <b>E–H</b>) Ventricular Prlhr-positive cilia (red) were double stained with GFAP (green) which stains a subtype of tanycytes. A partial population of the GFAP-positive tanycytes (green) possesses Prlhr-positive cilia (arrowheads). Nuclei were stained with DAPI (blue). V: third ventricles. Scale bars, 20 μm (the left panel in A; B, C, F, G), 10 μm (the right panel in A; H), 5 μm (D), and 40 μm (E).</p>
2015-06-08 03:08:08
NPFFR
drd
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2l
NMUR
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body weight control
Npy 2r encoding
transfected GPCR expression vectors
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