10.1371/journal.pone.0129308.g009
Pawel Bialk
Pawel
Bialk
Natalia Rivera-Torres
Natalia
Rivera-Torres
Bryan Strouse
Bryan
Strouse
Eric B. Kmiec
Eric
B. Kmiec
Sequence confirmation of ssODN/CRISPR edited cells.
Public Library of Science
2015
gene editing reaction
strand DNA breakage
Gene Editing Activity
crispr
ssodn
DNA cleavage activity
analysing reaction parameters
gene editing
guide RNA sequences
2015-06-08 02:45:41
Figure
https://plos.figshare.com/articles/figure/_Sequence_confirmation_of_ssODN_CRISPR_edited_cells_/1439848
<p>Unsynchronized HCT116-19 cells were electroporated under the following conditions; 2ug CRISPR 2C and 1.35ug 72NT at 5e5 cells/100ul. Cells were then sorted for GFP+ at 72 hours post electroporation. Immediately following cell sorting, DNA was isolated and the region surrounding the target base was amplified via PCR. Samples were submitted to Genewiz (South Plainfield, NJ) for sequencing analysis.</p>