10.1371/journal.pone.0127864.g004 Gregory Palardy Gregory Palardy Ajay B. Chitnis Ajay B. Chitnis Endocytosis of DeltaD point mutants. Public Library of Science 2015 mid bomb 1 Interaction Domain mib 1 Interaction Domain RING E 3 ligase zebrafish Notch ligand DeltaD neurogenic phenotype Zebrafish DeltaD Ubiquitylation 2015-05-28 03:35:38 Figure https://plos.figshare.com/articles/figure/_Endocytosis_of_DeltaD_point_mutants_/1429265 <p>Distribution of zdd2 (green) in COS7 cells transfected with DeltaD-NN (A), DeltaD-KK (E) and DeltaD-NN/KK following the internalization protocol described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127864#pone.0127864.g003" target="_blank">Fig 3</a>. Nuclei were labelled with DAPI (blue). Distribution of zdd2 (green) in COS7 cells co-transfected with DeltaD-NN (B-D), DeltaD-KK (F-H), DeltaD-NN/KK (J-L) and Mib1 following internalization as described above. Each set of 3 panels, respectively, shows distribution of the DeltaD construct (green), Myc-Mib1 (red)/nuclei (blue), and the merged image. (M) Summary of expression classes found in DeltaD full length, deletion and point mutants assayed following the internalization protocol described above. P-values for pairwise comparison based on Fisher’s Exact test of independence. P >. 05 does not meet the criteria for the Null hypothesis that pairs contain an equivalent distribution of expression classes.</p>